中文 
Technical training for Leica CM3600 XP - Whole Body Sectioning with GLP documentation
Date:2023-04-04
From March 27th to March 31st, 2023, I successfully graduated from the one week technical training for the large-scale whole body frozen slicer CM3600XP held at the Shenzhen Bay Laboratory.
The entire process: from configuring CMC → processing glass slides with solution A → anesthetizing SD rats by scraping and freezing embedding → operating slices on the machine → transferring samples with solution B and MTTS tape → HE staining → imaging.
Thank you for the strong support of Shenzhen Bay Laboratory, and thank you to all the teachers, Leica colleagues, and business friends who attended the learning session!
Review of Technical Training for Large Whole Body Freeze Slicer 3600XP
SD rats anesthetized, shaved and frozen for embedding
Rat patch, internal structure clearly visible
Slicing process, easy to operate, beginners can quickly get started
MTTS tape sample transfer printing
Pathological section H&E staining
Leica CM3600 XP is a frozen slicer for whole body sectioning, primarily used to examine the efficacy of labeled compounds, drugs, and biologics in quantitative preclinical studies. It can also be used for anatomical and morphological analysis of large objects, including low-temperature planning for 3D reconstruction.
For facilities that require slicing and experimental recording of large objects (such as those required for GLP), CM3600 XP is an ideal solution. Recording experimental parameters supports the reproducibility of all research institutions.
Data Tracking for GLP Compliance
Windows PC operated instrument controller allows to manage access rights and provides an audit trail through a chronological list of events and alarms. Experimental data are recorded in protocols that can be tailored to the laboratory's need.
Sectioning Large Objects
The heavy-duty microtome does not require fixation inside the stainless steel cryochamber and can be positioned to the user’s preference. The knife holder supports sectioning of specimens up to 450 x 150 x 200 mm (L,W,H) for very large objects.
Section Dehydration for QWBA
Quantitative whole-body autoradiography requires dehydration of frozen sections. The CM3600 XP features a twin compressor design to provide extra capacity for a constant cold air stream when used for dehydration.
Comfort and Safety
Sectioning large objects requires leaning into a large cryochamber. While this cannot be comfortable over longer time, Leica Biosystems did include some features for increased safety and comfort. A knee-lever supports hands-free sectioning so that both hands are free for capturing the section on tape. Safety measurements, such as a lid retainer that locks the open lid, or the use of 24V illumination inside the cryochamber reduce the risk of injuries.